Title:
【発明の名称】キメラ抗体の製造-組合せアプローチ
Document Type and Number:
Japanese Patent JPH06510671
Kind Code:
A
Abstract:
Methods are disclosed which may be used for the production of antibodies, or antibody fragments, which have the same binding specificity as a parent antibody but which have increased human characteristics. Humanised antibodies may be obtained by chain shuffling, perhaps using phage display technology. In one embodiment, a polypeptide comprising a heavy or light chain variable domain of a non-human antibody specific for an antigen of interest is combined with a repertoire of human complementary (light or heavy) chain variable domains. Hybrid pairings which are specific for the antigen of interest are selected. Human chains from the selected pairings may then be combined with a repertoire of human complementary variable domains (heavy or light) and humanised antibody polypeptide dimers can then be selected for binding specificity for antigen. The methods may be combined with CDR-imprinting. In another embodiment, component part of an antigen-binding site of a non-human antibody known to bind a particular antigen is combined with a repertoire of component parts of an antigen-binding site of human antibody, forming a library of antibody polypeptide dimers with antigen-binding sites. Hybrids selected from this library may be used in a second humanising shuffling step, or may already be of sufficient human character to be of value, perhaps after some modification to increase human character still further.
Inventors:
Hohenbaum, Hendricks Renels Jacobs Mateus
Michael, Bayer
Jesperth, Laurent Stefan Anteres
Winter, Gregory Paul
Michael, Bayer
Jesperth, Laurent Stefan Anteres
Winter, Gregory Paul
Application Number:
JP50593293A
Publication Date:
December 01, 1994
Filing Date:
September 23, 1992
Export Citation:
Assignee:
MEDICAL RES COUNCIL (GB)
CAMBRIDGE ANTIBODY TECH (GB)
CAMBRIDGE ANTIBODY TECH (GB)
International Classes:
A61K38/00; A61K39/395; A61K49/00; A61P35/00; C07K14/495; C07K14/00; C07K14/52; C07K16/00; C07K16/18; C07K16/22; C07K16/24; C07K16/28; C07K16/30; C07K16/40; C07K16/42; C07K16/46; C07K19/00; C12N5/10; C12N15/09; C12N15/10; C12N15/13; C12P21/08; C40B40/02; G01N33/531; (IPC1-7): C12P21/08; C07K13/00; C12N15/13; C12N15/62
Attorney, Agent or Firm:
Takashi Ishida (3 others)