Title:
【発明の名称】コクシジウムのモノクロ-ナル抗体
Document Type and Number:
Japanese Patent JPH0699475
Kind Code:
B2
Abstract:
A purified antigenic protein has been obtained which is capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix. The protein has a molecular weight of about 25,000 and is composed of two polypeptides joined by a disulfide bond. The two polypeptide subunits have molecular weights of about 17,000 and about 8,000, respectively. The gene encoding the protein has been sequenced and the amino acid sequence of the protein deduced therefrom and by direct peptide sequencing. The protein and antigenic polypeptides having an amino acid sequence included within the protein may be incorporated into a vaccine for conferring upon a chicken active immunity against infection by E. tenella and E. necatrix. A hybridoma cell line (ATCC No. HB8561) has been developed which produces a monoclonal antibody designated Ptn 7.2A4/4. This antibody may be used to confer upon a chicken specific passive protection against infection by E. tenella and E. necatrix. The antibody may also be used to obtain the purified protein antigen and the 11,500 and 6,500 dalton polypeptide fragments thereof. Finally, an anti-idiotype antibody to the Ptn. 7.2A4/4 monoclonal antibody may be prepared and used to confer upon a chicken active immunity against E. tenella and E. necatrix infection.
Inventors:
Karel Zetto Neumann, Giunia
Thomas Sea Gore
Jiyoung El Tedesco
Gary Earl Petersen
Randy Earl Simonson
Virginia Mellie Brothers
The Ames Gordon Files
Leland Shion Paul
Thomas Sea Gore
Jiyoung El Tedesco
Gary Earl Petersen
Randy Earl Simonson
Virginia Mellie Brothers
The Ames Gordon Files
Leland Shion Paul
Application Number:
JP12235585A
Publication Date:
December 07, 1994
Filing Date:
June 05, 1985
Export Citation:
Assignee:
Solvay (Societe Anonymous)
International Classes:
G01N33/569; A61K39/012; A61K39/395; C07K1/22; C07K14/00; C07K14/005; C07K14/195; C07K14/435; C07K14/44; C07K14/455; C07K16/00; C07K16/20; C07K16/42; C12N15/00; C12N15/02; C12N15/09; C12P21/00; C12P21/02; C12P21/08; G01N33/577; A61K38/00; A61K39/00; (IPC1-7): C07K13/00; A61K39/012; A61K39/395; C12N15/63; C12P21/02
Domestic Patent References:
| JP6067499A |
Attorney, Agent or Firm:
Akira Asamura (2 outside)
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