Title:
【発明の名称】ヒルジン誘導体、その取得法、組換えDNA及び組換えベクター
Document Type and Number:
Japanese Patent JPH07106157
Kind Code:
B2
Abstract:
A process for obtaining hirudin derivatives from E. coli secretor mutants in which (1) a recombinant vector on which the gene coding for a hirudin derivative is located directly behind a DNA section which codes for a bacterial signal peptide is constructed, (2) a E. coli secretor mutant is transformed with the recombinant vector constructed in step (1), (3) the transformed cells are cultivated in a medium and (4) the hirudin derivative is obtained from the medium, and a recombinant vector which contains one or more copies of a gene construct which codes for a protein consisting of a bacterial signal peptide and a hirudin derivative, and a hirudin derivative with the N-terminal amino-acid sequence A-Thr-Tyr-Thr-Asp in which A is Ala, Gln, His, Phe, Tyr, Glu, Ser, Asp or Asn are described.
Inventors:
Gerhard Schmid
Paul Harbelman
Paul Harbelman
Application Number:
JP5671491A
Publication Date:
November 15, 1995
Filing Date:
March 20, 1991
Export Citation:
Assignee:
Consortium Fuyuru Electro Chemistry Industrie Geselsyaft Mitsubeshiyu Renktel Haftsung
International Classes:
A61P7/02; C07K14/005; C07K14/815; C12N1/21; C12N9/10; C07K14/195; C12N15/00; C12N15/09; C12N15/12; C12N15/15; C12N15/71; C12N15/72; C12P21/02; C12R1/19; (IPC1-7): C12P21/02; C07K14/815; C12N15/09
Domestic Patent References:
| JP235077A |
Other References:
【文献】欧州特許出願公開352228(EP,A)
【文献】欧州特許出願公開356335(EP,A)
【文献】欧州特許出願公開356335(EP,A)
Attorney, Agent or Firm:
Toshio Yano (2 outside)