Title:
【発明の名称】トリプトファン・プロモーター―オペレーターを用いる細菌によるポリペプチドの発現用ベクター
Document Type and Number:
Japanese Patent JPH0724582
Kind Code:
B2
Abstract:
Novel plasmidic expression vehicles are provided for the production in bacteria of heterologous polypeptide products, the vehicles having a sequence of double-stranded DNA comprising, in phase from a first 5 min to a second 3 min end of the coding strand, a trp promoter-operator, nucleotides coding for the trp leader ribosome binding site, and nucleotides encoding translation initiation for expression of a structural gene that encodes the amino acid sequence of the heterologous polypeptide. The DNA sequence referred to contains neither a trp attenuator region nor nucleotides coding for the trp E ribosome binding site. Instead, the trp leader ribosome binding site is efficiently used to effect expression of the information encoded by an inserted gene.
Inventors:
DENNISU JII KUREIDO
DANIERU JII YANSUURA
HAABAATO ERU HEINETSUKAA
GIUSETSUPE EFU MIOZARI
DANIERU JII YANSUURA
HAABAATO ERU HEINETSUKAA
GIUSETSUPE EFU MIOZARI
Application Number:
JP4052981A
Publication Date:
March 22, 1995
Filing Date:
March 23, 1981
Export Citation:
Assignee:
GENENTECH INC
International Classes:
A61K38/00; A61K38/27; C12N15/00; A61K38/28; A61K39/00; A61K39/395; C07D249/08; C07D253/06; C07D501/20; C07H21/00; C07H21/04; C07K14/61; C07K14/655; C12N1/20; C12N15/09; C12N15/10; C12N15/62; C12N15/66; C12N15/71; C12P19/34; C12P21/00; C12Q1/68; C12N; (IPC1-7): C12N15/09
Domestic Patent References:
| JPS54163600A |
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)
Next Patent: ロボットハンド機構部における位置決めガイドピン