Title:
【発明の名称】ポリスチレン表面に核酸プローブを固定化する方法
Document Type and Number:
Japanese Patent JPH07502656
Kind Code:
A
Abstract:
Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water, (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface, and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent, and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.
Inventors:
Sheridan, patrick
Chang, Chu-Ann
Running, joyce
Chang, Chu-Ann
Running, joyce
Application Number:
JP51195693A
Publication Date:
March 23, 1995
Filing Date:
December 22, 1992
Export Citation:
Assignee:
Bayer corporation
International Classes:
C07H21/04; C12Q1/68; C12Q1/70; G01N33/53; G01N33/547; G01N33/566; C12N15/09; C12R1/92; (IPC1-7): C12Q1/68; C12Q1/70; C12N15/09; C07H21/04
Attorney, Agent or Firm:
Hidesaku Yamamoto