Title:
【発明の名称】蛍光性沈澱を生じる基質を用いる酵素分析
Document Type and Number:
Japanese Patent JPH08500961
Kind Code:
A
Abstract:
Activity of enzymes and enzyme conjugates is detected using novel substrates made from a class of fluorophores, generally including quinazolinones (quinazolones), benzimidazoles, benzothiazoles, benzoxazoles, quinolines, indolines, and phenanthridines, having the general formula: where carbon atoms of -C1=C2- are joined to complete a first 5- or 6-membered aromatic ring which optionally contains one of the hetero atoms N, O or S, and carbon atoms of -C4-N=C3- are joined to complete a second 5- or 6-membered aromatic ring that contains a nitrogen between C3 and C4 and optionally contains an additional hetero atom N, O or S, where the first and second aromatic rings may be joined by a 5- or 6-membered bridging ring that contains at least the C2 from the first aromatic ring and the C3 from the second aromatic ring, where each of the first and second aromatic rings may be fused to at least one additional aromatic ring that may contain at least one of the hetero atoms N, O or S, and where each of said aromatic rings may be further modified by substitution of any hydrogens on an aromatic carbon by substituents that are halogen, nitro, cyano, aryl, lower alkyl (1-4 carbons), perfluoroalkyl (1-4 carbons), or alkoxy (1-4 carbons), or any combination thereof; and the fluorophore is covalently linked to a blocking group through an oxygen -O- at C1; such that removal of the blocking group by enzyme activity yields a precipitate.
Inventors:
Hoogland, Richard Pea
Han, Yuphone
You, Stephen
Terpets Knig, Ewald
Olson, Nells
Nailway, John
Karen Larrison
Han, Heian
Han, Yuphone
You, Stephen
Terpets Knig, Ewald
Olson, Nells
Nailway, John
Karen Larrison
Han, Heian
Application Number:
JP50454693A
Publication Date:
February 06, 1996
Filing Date:
August 21, 1992
Export Citation:
Assignee:
Molecular Probes Inc
International Classes:
C07D215/14; C07D235/18; C07D239/91; C07D263/56; C07D263/57; C07D277/64; C07D277/66; C07F9/6512; C07F9/6541; C07H15/04; C07H15/26; C07K4/00; C08B37/00; C12Q1/34; C12Q1/42; C12Q1/44; C12Q1/68; C12Q1/00; (IPC1-7): C12Q1/00; C12Q1/34; C12Q1/42; C12Q1/44
Attorney, Agent or Firm:
Kyozo Yuasa (6 people outside)