Title:
【発明の名称】血液から核酸の製造
Document Type and Number:
Japanese Patent JPH08501208
Kind Code:
A
Abstract:
The present invention provides a method and kit for preparation of nucleic acid from white blood cells for detection and/or for amplification. The nucleic acid may be prepared by contacting a sample of cells, particularly whole blood, with an appropriate lysing agent under conditions in which red blood cells are lysed but white bloods cells are not. The treated sample may be centrifuged so as to separate desired white blood cells from non-desired red blood cells, and then purifying and lysing said desired white blood cells. The nucleic acid associated with the lysed cells can be detected and/or amplified and the nucleic acid can also be screened for a certain nucleic acid sequence, such as a viral nucleic acid sequence.
Inventors:
Rider, thomas bee
Daniel Kell
Daniel Kell
Application Number:
JP50177193A
Publication Date:
February 13, 1996
Filing Date:
June 11, 1993
Export Citation:
Assignee:
Gen-Probe Incorporated
International Classes:
C12N15/09; C12N1/06; C12N15/10; C12Q1/68; C12Q1/70; G01N33/50; (IPC1-7): C12Q1/68; C12Q1/70
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)