【発明の名称】ヒトシクロオキシゲナーゼ-2cDNA及びシクロオキシゲナーゼ-2の阻害を評価するアッセイ

Title:

【発明の名称】ヒトシクロオキシゲナーゼ-2cDNA及びシクロオキシゲナーゼ-2の阻害を評価するアッセイ

Document Type and Number:

Japanese Patent JPH08504584

Kind Code:

A

Abstract:

An assay for determining the cyclooxygenase-2 activity of a sample comprising the steps of: (a) adding (1) a human osteosarcoma cell preparation, (2) a sample, said sample comprising a putative cyclooxygenase-2 inhibitor, and (3) arachidonic acid; and (b) determining the amount of prostaglandin E2 produced in step (a).

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Inventors:

Chrome rishiyu, wanda ah
Kennedy, Brian Pee
O'neal, gear
Bitzkel, Philip J
Won, Elisabeth
Mancini, Joseph Ah

Application Number:

JP51463294A

Publication Date:

May 21, 1996

Filing Date:

December 17, 1993

Export Citation:

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Assignee:

Merck Frost Canada Inc.

International Classes:

C12N15/09; C12N5/10; C12N9/02; C12Q1/26; C12Q1/48; C12Q1/61; G01N33/50; G01N33/88; (IPC1-7): C12N15/09; C12Q1/26; C12N9/02; C12N5/10

Attorney, Agent or Firm:

Yoshio Kawaguchi (2 outside)

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