Title:
【発明の名称】核酸のコピーの産生
Document Type and Number:
Japanese Patent JPH10500862
Kind Code:
A
Abstract:
Amplification of nucleic acids using oligonucleotides immobilized on solid phase supports is disclosed. Target nucleic acid strands (in a sample to be analyzed) are hybridized to the oligonucleotides and copy target strands (incorporating immobilized oligonucleotide) are produced using the target strands as templates. The target strands are denatured from the copy target strands and rehybridized to non-extended oligonucleotides. In the next step, new copy target 1 strands are synthesized from the oligonucleotides hybridized to the target strands, and simultaneously copy target 2 strands are synthesized using previously generated copy target 1 strands. Sequence of denaturing, rehybridizing, and producing copy target 1 and copy target 2 strands is repeated as often as necessary to give the desired degree of amplification.
Inventors:
Stephen John, Minter
Application Number:
JP50051296A
Publication Date:
January 27, 1998
Filing Date:
May 30, 1995
Export Citation:
Assignee:
Tepnel Medical Limited
International Classes:
C12Q1/68; C12N15/09; (IPC1-7): C12Q1/68; C12N15/09
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)