Title:
【発明の名称】B型肝炎ウイルスのXタンパク質の転写促進活性の阻害方法
Document Type and Number:
Japanese Patent JPH11503303
Kind Code:
A
Abstract:
Disclosed is a method for screening for inhibitors of Hepatitis B Virus pX activity. The method involves contacting a test compound with (I) the pX protein of HBV, (ii) a transcription factor comprising the bZIP domain, or fragments that comprise a minimal a bZIP domain, and (iii) an oligoduplex comprising a target DNA sequence of the transcription factor to form a test mixture. After incubating the test mixture under appropriate conditions and for a sufficient time to allow pX-mediated dimerization and DNA binding of the transcription factor to occur, the level of DNA binding of the transcription factor in each test mixture is determined. A test compound is considered to be any compound that causes a decrease in the level of DNA binding in the test mixture relative to the level of DNA binding in control mixtures.
Inventors:
Green, michael di
Perini, Giovanni
Lily, james
Perini, Giovanni
Lily, james
Application Number:
JP51784596A
Publication Date:
March 26, 1999
Filing Date:
December 06, 1995
Export Citation:
Assignee:
Scriptgen Pharmaceuticals, Inc.
University of Massachusetts Medical Center
University of Massachusetts Medical Center
International Classes:
G01N33/576; C07K14/02; C12N15/00; C12N15/09; C12N15/67; C12Q1/18; C12Q1/68; G01N33/15; C12R1/92; (IPC1-7): C12N15/09; C12Q1/68; G01N33/15; G01N33/576
Attorney, Agent or Firm:
Masatake Shiga (2 outside)