Title:
【発明の名称】ヘルカーゼ活性および改善された溶解性を有するHCV NS3タンパク質フラグメント
Document Type and Number:
Japanese Patent JPH11512606
Kind Code:
A
Abstract:
The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg2+ and Mn2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.
Inventors:
Han, Jean
Cho, June Ho
Cho, June Ho
Application Number:
JP51347897A
Publication Date:
November 02, 1999
Filing Date:
September 12, 1996
Export Citation:
Assignee:
Chiron Corporation
International Classes:
A61K38/00; A61P31/20; A61P43/00; C07K14/00; C07K14/18; C07K19/00; C12N1/21; C12N15/09; C12N9/00; C12N9/02; C12N9/22; C12N9/50; C12N9/90; C12N15/00; C12N15/57; C12R1/19; C12R1/92; (IPC1-7): C12N9/22; C07K19/00; C12N15/09
Domestic Patent References:
| JPH05508219A | 1993-11-18 | |||
| JPH06319583A | 1994-11-22 |
Attorney, Agent or Firm:
Shusaku Yamamoto