Document Type and Number:
Japanese Patent JPS56500442
Kind Code:
A
Abstract:
A controlled protein fractionation method in which a cold protein-containing solution is circulated in contact with one side of a membrane and the temperature is controlled by circulating a protein precipitant solution in contact with the opposite side of the membrane. The flow rate is increased and back pressure is exerted on the precipitant solution to cause it to penetrate the membrane, mix with the protein solution and precipitate protein in fine particles along an inside membrane wall. The precipitated protein may be concentrated and separated by centrifugation or diafiltration. The membrane is preferably in the form of a plurality of hollow fibers. Control of process variables increases yield and purity, decreases processing time and reduces degree of denaturization. The method is especially applicable to carrying out the Cohn cold alcohol fractionation process.
Application Number:
JP50127480A
Publication Date:
April 09, 1981
Filing Date:
May 01, 1980
Export Citation:
International Classes:
C07K1/30; C07K1/34; B01D21/01; C07K14/00; C07K14/435; C07K14/76; C07K14/765; (IPC1-7): C07G7/00; B01D13/00