Document Type and Number:
Japanese Patent JPS57501610
Kind Code:
A
Abstract:
Method for carrying out biological and chemical conversion processes which require the presence of one or more catalytically active substances, such as enzymes, microorganisms, cells, organelles, cell homogenates, and organic or inorganic catalysts. The method is carried out in a liquid system consisting of at least two aqueous phases (F1, F2) so selected that the catalytic substance (K) is enriched in one (F1) of the phases, while the starting substrate (S) of the process is distributed in both phases or, advantageously, is enriched in the same phases as the catalytic substance, and the process product (P) is distributed in both phases or, advantageously, is enriched in the other phase (F2). During the process, the system is agitated to finely disperse one phase in the other. The process product can be recovered from the other phase, either by ceasing to agitate the system and allowing the phases to separate, or by maintaining dispersement of one phase in only a part of the other phase and continuously tapping off the upper part of the other phase for removal of the process product therefrom and then returning the removed phase part to the agitated part of the liquid system. Advantageously, the other phase (E2) of the liquid system has a substantially larger volume than the mentioned one phase (F1). The method can be applied with gaseous, low-molecular, macromolecular and particulate starting substrates, which may be soluble or insoluble in the system. The method according to the invention affords particular advantages when carrying out enzymatic conversion reactions requiring the presence of a coenzyme. In this case, the coenzyme and an enzyme operable to regenerate the coenzyme are also introduced to the system, the two phases of which are selected so that the coenzyme and the further enzyme are also enriched in the same phase as the enzyme requisite to the conversion reaction.
Application Number:
JP50334881A
Publication Date:
September 09, 1982
Filing Date:
October 16, 1981
Export Citation:
International Classes:
B01J14/00; C02F1/26; C02F3/12; C02F3/34; C12J1/00; C12M1/40; C12N9/28; C12P1/00; C12P7/06; C12P7/28; C12P7/54; C12P7/62; C12P19/14; C12P37/00; C12P37/06; C13K1/00; (IPC1-7): C12P1/00; C13K1/00