PURPOSE: To obtain a recombinant DNA capable of multiplying a human interferon-β in bacteria and eucaryotic cells in a large amount, by taking out a DNA containing the human interferon-β gene directly from a human chromosomal gene.
CONSTITUTION: DNA extracted from a human whole chromosomal DNA, e.g. human fetal liver, is partially digested with a restriction enzyme, e.g. Hae III or AluI, and fragmented to a suitable length. The resultant fragments are directly or a part of suitable length is only taken out, concentrated by the electrophoretic method, etc. and inserted into a vector DNA by the recombinant DNA technique to give the aimed recombinant DNA. The recombinant DNA is searched by using a recombinant DNA, having DNA exhibiting the complementation to a human interferon mDNA, and labeled with a radioisotope as a probe, and the aimed recombinant DNA containing the total transfer region of the human interferon-β gene and further the region taking no part in the adjustment of the transfer is collected.
TANIGUCHI FUSATSUGU
OONO SHIGEO
| JPS57130998A | 1982-08-13 |