Title:
【発明の名称】融合リポソ-ムおよびリポソ-ムの酸誘導融合方法
Document Type and Number:
Japanese Patent JPS61501897
Kind Code:
A
Abstract:
Liposomes containing phosphatidylethanolamine, palmitoyl homocysteine or oleic acid or palmitic acid, fuse rapidly when the pH of the medium is reduced below 7. Liposome fusion was measured by (a) mixing of the liposomal lipids as shown by resonance energy transfer, (b) gel filtration and (c) electron microscopy. The presence of phosphatidylethanolamine or acid addition esters thereof in the liposomes greatly enhances fusion; whereas the presence of phosphatidylcholine inhibits fusion. During fusion of liposomes containing phosphatidylethanolamine:palmitoyl homocysteine (8:2), almost all of the encapsulate calcein is released. Inclusion of cholesterol (40%) in the liposomes substantially decreases leakage without impairing fusion. Those pH sensitive liposomes are fused to deliver biologically active molecules such as DNA, into living cells.
Inventors:
Juan, Leaf
Connor, The Erome
Connor, The Erome
Application Number:
JP50186085A
Publication Date:
September 04, 1986
Filing Date:
April 09, 1985
Export Citation:
Assignee:
University-City of Tennessee-Lisa-Co-Polation
International Classes:
A01H1/00; A01H5/00; A61K9/00; A61K9/127; B01J13/02; C12N5/00; C12N5/02; C12N5/10; C12N15/00; C12N15/88; (IPC1-7): A01H1/00; A01H5/00; A61K9/10; B01J13/02; C12N5/00; C12N15/00
Domestic Patent References:
| JPS58152812A | 1983-09-10 | |||
| JPS60155109A | 1985-08-15 |
Attorney, Agent or Firm:
Kyozo Yuasa