Document Type and Number:
Japanese Patent JPWO2022030378
Kind Code:
A1
Abstract:
An object is to provide a technique for detecting a test substance more easily using a nucleic acid structure. The object is achieved by a nucleic acid structure comprising a partial nucleic acid structure 1 and a partial nucleic acid structure 2, the partial nucleic acid structure 1 and the partial nucleic acid structure 2 being linked by a Holliday junction, the partial nucleic acid structure 1 comprising a protruding structure 1a containing a nucleic acid aptamer sequence, and a protruding structure 1b containing a split domain 1 of an enzyme, and the partial nucleic acid structure 2 comprising a protruding structure 2a containing a sequence complementary to part or all of the nucleic acid aptamer sequence, and a protruding structure 2b containing a split domain 2 paired with the split domain 1.
Application Number:
JP2022541500A
Publication Date:
February 10, 2022
Filing Date:
July 30, 2021
Export Citation:
Other References:
WALTER HK ET AL.: ""DNA Origami Traffic Lights" with a Split Aptamer Sensor for a Bicolor Fluorescence Readout", NANO LETT., 2017, 17(4), PP.2467-2472, JPN6021036213, ISSN: 0005055035
HOU T ET AL.: "Label-free colorimetric detection of coralyne utilizing peroxidase-like split G-quadruplex DNAzyme", ANAL. METHODS, 2013, 5(18), PP.4671-4674, JPN6021036212, ISSN: 0005055036
KUZUYA A ET AL.: "Nanomechanical DNA origami 'single-molecule beacons' directly imaged by atomic force microscopy", NAT. COMMUN., 2011, 2:449, JPN6021036211, ISSN: 0005055037
HOU T ET AL.: "Label-free colorimetric detection of coralyne utilizing peroxidase-like split G-quadruplex DNAzyme", ANAL. METHODS, 2013, 5(18), PP.4671-4674, JPN6021036212, ISSN: 0005055036
KUZUYA A ET AL.: "Nanomechanical DNA origami 'single-molecule beacons' directly imaged by atomic force microscopy", NAT. COMMUN., 2011, 2:449, JPN6021036211, ISSN: 0005055037