Title:
METHOD FOR PRODUCTION OF HIGHLY PURE PHOSPHOLIPID, AND HIGHLY PURE SPHINGOMYELIN AND PLASMALOGEN-TYPE GLYCEROPHOSPHOLIPID PRODUCED BY THE METHOD
Document Type and Number:
WIPO Patent Application WO/2009/154309
Kind Code:
A1
Abstract:
Disclosed is a method for producing a highly pure sphingomyelin and a highly pure plasmalogen-type glycerophospholipid from a biological material in high yields by simple manipulations. Specifically disclosed is a method for producing highly pure phospholipids, which comprises the following steps (A) to (E): (A) subjecting a dried total lipid content extracted from a biological material to an extraction treatment with a specific mixed solvent to separate the dried total lipid content into an insoluble fraction and a soluble fraction; (B) washing the insoluble fraction obtained in the step (A) with a specific mixed solvent to produce a crude sphingomyelin; (C) washing the soluble fraction obtained in the step (A) with a water-soluble ketone solvent to produce a crude plasmalogen-type glycerophospholipid; (D) reacting the crude sphingomyelin obtained in the step (B) with an enzyme to produce a sphingomyelin having a purity of 90% or higher; and (E) reacting the crude plasmalogen-type glycerophospholipid obtained in the step (C) with an enzyme to produce a plasmalogen-type glycerophospholipid having a purity of 40% or higher.
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Inventors:
NADACHI YOSHITAKA (JP)
MAWATARL SIRO (JP)
MAWATARL SIRO (JP)
Application Number:
PCT/JP2009/061666
Publication Date:
December 23, 2009
Filing Date:
June 19, 2009
Export Citation:
Assignee:
UMEDA JIMUSHO LTD (JP)
NADACHI YOSHITAKA (JP)
MAWATARL SIRO (JP)
NADACHI YOSHITAKA (JP)
MAWATARL SIRO (JP)
International Classes:
A23J7/00; C11B11/00; C11B1/10; C11B3/00; C11B3/02
Domestic Patent References:
| WO2008146942A1 | 2008-12-04 |
Foreign References:
| KR100225669B1 | 1999-10-15 | |||
| JPH0439398A | 1992-02-10 | |||
| JP2008179588A | 2008-08-07 |
Other References:
FOGERTY, A. C.: "The composition of the fatty acids and aldehydes of the ethanolamine and choline phospholipids of various meats", INT J FOOD SCI TECHNOL, vol. 26, no. 4, 1991, pages 363 - 371, XP003027630
AGGERBECK, LAWRENCE P.: "Enzymatic Probes of Lipoprotein Structure", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 251, no. 12, 1976, pages 3823 - 3830, XP003027631
NAKANISHI, TAKEO: "Shokuniku no Rin Shishitsu ni Kansuru Kenkyu", NICCHIKU KAIHO, vol. 37, no. 1, 1966, pages 7 - 14, XP008142966
YUNOKI, KEITA: "Separation and Determination of Functional Complex Lipids from Chicken Skin", J. AM. OIL CHEM. SOC., vol. 85, 2008, pages 427 - 433, XP008140811
"Oya Dori Miriyo Bui kara no Sphingomyelin to Plasmalogen no Bunri to Bunseki", JAPAN OIL CHEMISTS' SOCIETY DAI 47 KAI NENKAI KOEN YOSHISHU, 17 September 2008 (2008-09-17), pages 232, XP008140872
J. LIPID RES., vol. 44, 2003, pages 164 - 171
J. MOL. NEUROSCI., vol. 16, 2001, pages 263 - 272
See also references of EP 2308954A4
AGGERBECK, LAWRENCE P.: "Enzymatic Probes of Lipoprotein Structure", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 251, no. 12, 1976, pages 3823 - 3830, XP003027631
NAKANISHI, TAKEO: "Shokuniku no Rin Shishitsu ni Kansuru Kenkyu", NICCHIKU KAIHO, vol. 37, no. 1, 1966, pages 7 - 14, XP008142966
YUNOKI, KEITA: "Separation and Determination of Functional Complex Lipids from Chicken Skin", J. AM. OIL CHEM. SOC., vol. 85, 2008, pages 427 - 433, XP008140811
"Oya Dori Miriyo Bui kara no Sphingomyelin to Plasmalogen no Bunri to Bunseki", JAPAN OIL CHEMISTS' SOCIETY DAI 47 KAI NENKAI KOEN YOSHISHU, 17 September 2008 (2008-09-17), pages 232, XP008140872
J. LIPID RES., vol. 44, 2003, pages 164 - 171
J. MOL. NEUROSCI., vol. 16, 2001, pages 263 - 272
See also references of EP 2308954A4
Attorney, Agent or Firm:
NAKAMURA, Shizuo (ST Bldg. 24-10, Taito 2-chome, Taito-ku, Tokyo 16, JP)
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