Title:
2,5-DKGパーミアーゼ
Document Type and Number:
Japanese Patent JP4975937
Kind Code:
B2
Abstract:
A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2-KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.
Inventors:
Dartis, Veronique A
Hotch, James A.
Barre, Fernando
Kumar, Manoi
Hotch, James A.
Barre, Fernando
Kumar, Manoi
Application Number:
JP2002517759A
Publication Date:
July 11, 2012
Filing Date:
August 03, 2001
Export Citation:
Assignee:
Microgenomics Incorporated
Danisco USA Inc.
Danisco USA Inc.
International Classes:
C12N15/09; C07K14/24; C07K14/26; C07K16/40; C12N1/21; C12N9/14; C12N15/31; C12P7/60
Domestic Patent References:
| JP2004519214A |
Foreign References:
| WO2000022170A1 |
Other References:
Mol. Microbiol., 37[3](2000) p.561-573
Attorney, Agent or Firm:
Kazuo Shamoto
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita