PURPOSE: To precisely quantify a 3-deoxyglucosone derivative in a biological sample by using the 3-deoxyglucosone derivative marked with 13C or 14C as an internal marker material for gas chromatographic mass spectrometry.
CONSTITUTION: A 3-deoxyglucosone derivative synthesized with the glucose marked by 13C or 14C in advance as a departure point and expressed by the formula and its methoxyoxime body are used as an internal marker material for gas chromatographic mass spectrometry, where *C indicates 13C or 14C, X indicates O or N-OR (R is Me, Et, or H), and Y indicates Side3, or SiMe2, or SiMe2tBu. The 3-deoxyglucosone derivative having been hardly detected from the biological tissue such as blood or urine due to its instability and reactivity can be precisely detected by this method.
NIIMURA KOICHI
OHARA MINORU
TOMIYAMA SHIGEMI
JPH04145360A | 1992-05-19 |