To provide a 5-oxo-ETE receptor protein discovering an in vivo signal transduction mechanism or identifying a new drug target protein, its gene and a 5-oxo-ETE receptor agonist or antagonist.
A plurality of genes considered to encode a G-protein coupled receptor from a human genome sequence are searched and some thereof are cloned. Fusion genes of α-subunit genes of Gi1 bound to the 3'-terminal parts of the cloned genes are prepared and the resultant fusion genes are then transduced into insect cells to express receptor-Gi1α fusion proteins. When an agonist is bound to the receptor part of the fusion proteins, the binding of [35S]-GTPγS to the Gi1α part thereof can be expected from properties of the receptor and G protein. A search is made for whether or not a fusion protein having the bonds of the [35S]-GTPγS increased in a dose-dependent manner of the 5-oxo-ETE is present in the prepared fusion proteins.
TAKEDA SHIGEKI
YAMAMOTO ATSUSHI
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