Title:
グルコサミニルの6-O硫酸化方法及び6-O硫酸化された多糖製剤
Document Type and Number:
Japanese Patent JP2005507640
Kind Code:
A
Abstract:
Disclosed are methods of 6-O sulfating glucosaminyl N-acetylglucosamine residues (GlcNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations made by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided.
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Inventors:
Rosenberg, Robert Dee
Zhang, Liguan
Beer, David El
Zhang, Liguan
Beer, David El
Application Number:
JP2002577881A
Publication Date:
March 24, 2005
Filing Date:
March 28, 2002
Export Citation:
Assignee:
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
International Classes:
A61K8/73; A61Q5/12; A61Q19/00; C08B37/00; C12N5/10; C12N15/09; C12N9/10; C12P19/26; C12Q1/02; C12Q1/68; (IPC1-7): C12P19/26; C12N5/10; C12N15/09; C12Q1/02; C12Q1/68
Attorney, Agent or Firm:
Yoichi Oshima