To provide adeno-associated virus(AAV) materials and methods which are useful for DNA delivery to cells, more particularly to provide recombinant AAV(rAAV) genomes, to provide methods for packaging rAAV genomes, to provide stable host cell lines producing rAAV, and to provide methods for delivering genes of interests to cells utilizing the rAAV.

A DNA vector comprising a recombinant adeno-associated virus genome and DNA sequences encoding adeno-associated rep/cap proteins, wherein the recombinant adeno-associated virus genome comprises adeno- associated virus inverted terminal repeats flanking non-adeno-associated virus DNA sequences operably linked to promoter and polyadenylation sequences and wherein the DNA sequences encoding rep/cap proteins are located outside the genome.