Title:
アフィニティークロマトグラフ装置
Document Type and Number:
Japanese Patent JP6788028
Kind Code:
B2
Abstract:
The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies' include (1) at least one polymer membrane that contains therein inorganic particles and (2) at least one impermeable layer (e.g., a thermoplastic polymer in a solid state). The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices has a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
Inventors:
Michael Sea. McManaway
Brian Jay Swetlin
Kenneth S. The Co
Brian Jay Swetlin
Kenneth S. The Co
Application Number:
JP2018552179A
Publication Date:
November 18, 2020
Filing Date:
March 29, 2017
Export Citation:
Assignee:
W.L. GORE & ASSOCIATES, INCORPORATED
International Classes:
B01J20/281; B01D15/38; B01J20/282; B01J20/283; C12M1/34; G01N30/88
Domestic Patent References:
| JP5509405A | ||||
| JP63072699A | ||||
| JP2071837A | ||||
| JP1114746A | ||||
| JP9234097A | ||||
| JP2001343349A | ||||
| JP2004245734A | ||||
| JP2015190939A | ||||
| JP2012504233A |
Foreign References:
| US20050115890 | ||||
| US20150253280 |
Attorney, Agent or Firm:
Atsushi Aoki
Shinji Mitsuhashi
Satoshi Deno
Atsushi Ebiya
Naori Kota
Tatsunori Ikeda
Shinji Mitsuhashi
Satoshi Deno
Atsushi Ebiya
Naori Kota
Tatsunori Ikeda