Title:
AFFINITY COLUMN CHROMATOGRAPHY USING BIOTINYLATED LITHOCHOLIC ACID
Document Type and Number:
Japanese Patent JP2003159052
Kind Code:
A
Abstract:
To provide an affinity column chromatography for isolation, selection of an enzyme included in a material, in an inexpensive and simple operation.
The affinity column chromatography uses an immobilized carrier obtained by immobilizing a biotinylation product of an inhibitor to the enzyme through a Linker bonding.
Inventors:
TANAKA MANAMI
SAKAGUCHI KENGO
SUGAWARA FUMIO
SAKAGUCHI KENGO
SUGAWARA FUMIO
Application Number:
JP2001358349A
Publication Date:
June 03, 2003
Filing Date:
November 22, 2001
Export Citation:
Assignee:
NAT INST OF ADV IND & TECHNOL
NIPPON FLOUR MILLS
NIPPN TECHNOCLUSTER INC
NIPPON FLOUR MILLS
NIPPN TECHNOCLUSTER INC
International Classes:
C12N9/00; B01D15/08; B01J20/26; B01J20/34; C07J43/00; C12N9/99; (IPC1-7): C12N9/00; B01D15/08; B01J20/26; B01J20/34; C07J43/00; C12N9/99
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)
Previous Patent: MARINE BACTERIA RESISTANT TO NICKEL
Next Patent: METHOD FOR ACTIVATING PLASMA PREKALLIKREIN
Next Patent: METHOD FOR ACTIVATING PLASMA PREKALLIKREIN