PURPOSE: To perform the induction and growth of an indefinite embryo in high efficiency by adding filtrate of microalgae culture liquid and/or extract of microalgae as an indefinite embryo growth promoter to a basal medium and culturing a plant tissue or cultured cell in the medium.
CONSTITUTION: Microalgae such as Chlamydomonas dorsobentralis or Chlorella vulgaris are cultured and the product is subjected to centrifugal separation or filtration, etc. The obtained filtrate of cultured microalgae and/or the cells are extracted by contacting with an aqueous solvent. The extract is used as an indefinite embryo growth promoter and added to a basal medium such as Murashige-Skoog medium. A plant tissue or cultured cell is cultured in the medium to enable the efficient reproduction of a plant body via an indefinite embryo.
ONO MAYUMI
HISHINUMA KIYOSHI
UMETSU HIRONORI
MATSUNAGA TADASHI
MATSUNAGA TADASHI
Next Patent: AGENT FOR PROMOTING GERMINATION OF ARTIFICIAL SEED