Title:
ALCOHOL ACETYLTRANSFERASE GENE AND ITS USE
Document Type and Number:
Japanese Patent JP3363197
Kind Code:
B2
Abstract:
PURPOSE: To enable production of an alcoholic beverage enriched in an ester flavor by fermenting a saccharide in the presence of a yeast-derived alcohol acetyltransferase exhibiting an acetic acid ester production activity.
CONSTITUTION: A plasmid pUc4k containing a G418-resistant gene is cut by using a restriction enzyme Sa1I and the resultant fragment containing the G418- resistant gene is bonded to YATK11 cut by Sa1I to obtain a vector YATK11 for introducing an alcohol acetyltransferase(AATase) gene into sake yeast. After transforming a yeast for production of an alcoholic beverage using the vector YATK11G, the resultant transformant is cultured in a suitable culture medium and the generated AATase is recovered. This AATase exhibits an acetic acid ester production activity through a reaction in which an acetyl group is transferred through an acetyl CoA to alcohol and an alcoholic beverage enriched in an ester flavor can be produced by fermenting a saccharide in the presence of this AATase.
Inventors:
Toshi Fujii
Iwamatsu Akihiko
Hiroyuki Zenmoto
Miki Toshitaka
Takayuki Bogaki
Nagasawa Nao
Iwamatsu Akihiko
Hiroyuki Zenmoto
Miki Toshitaka
Takayuki Bogaki
Nagasawa Nao
Application Number:
JP6299793A
Publication Date:
January 08, 2003
Filing Date:
February 26, 1993
Export Citation:
Assignee:
Kirin Brewery Co., Ltd.
Ozeki Co., Ltd.
Ozeki Co., Ltd.
International Classes:
C12C11/00; C12C11/02; C12G1/022; C12G3/02; C12N1/19; C12N9/10; C12N15/09; C12N15/54; C12G1/02; C12R1/865; (IPC1-7): C12N9/10; C12G3/02; C12N1/19; C12N15/09
Foreign References:
Attorney, Agent or Firm:
Kazuo Sato (2 outside)
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