Title:
【発明の名称】アミノポリオールアミンオキシダーゼポリヌクレオチド類および関連のポリペプチド類ならびに使用方法
Document Type and Number:
Japanese Patent JP2002520058
Kind Code:
A
Abstract:
The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
Inventors:
Jonathan P. Duvic
Jacob T. Gilliam
Joyce Earl Maddox
Jacob T. Gilliam
Joyce Earl Maddox
Application Number:
JP2000560257A
Publication Date:
July 09, 2002
Filing Date:
July 08, 1999
Export Citation:
Assignee:
Pioneer Hi-Bread International, Inc.
International Classes:
A01N63/02; A23K1/00; A23K1/165; A23K3/03; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12N9/06; C12N9/16; A01H5/00; C12N15/09; C12N15/31; C12N15/53; C12N15/82; C12Q1/26; (IPC1-7): C12N15/09; A01H5/00; A01N63/02; A23K1/165; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12Q1/26
Attorney, Agent or Firm:
Chika Takagi (2 outside)