To provide a process for diploid anchorage-dependent cell culture of an animal in the absence of exogenous components of primary animal origin, and to provide a cell culture medium substantially free of the exogenous components of the primary animal origin suitable for carrying out the process.
There are provided the process for diploid anchorage-dependent cell culture of the animal, preferably a human in the absence of the exogenous components of the primary animal origin, and the cell culture medium substantially free of the exogenous components of the primary animal origin suitable for carrying out the process. In particular, there is provided the cell culture medium comprising at least one, more preferably several exogenous animal-free growth factors. Furthermore, there are provided a process for culturing animal, preferably human diploid anchorage-dependent cells in the culture medium, involving the use of a trypsin substitute of non-animal origin for passaging the cells, and further a process for producing viruses, viral vaccines etc.
GHISLAIN YVES JULES MAURICE
GONZE MARIE-MONIQUE JANE
KNOTT ISABELLE SOLANGE LUCIE
MAGGETTO CARINE
| JP2002520014A | 2002-07-09 | |||
| JPH0748277A | 1995-02-21 | |||
| JP2001501830A | 2001-02-13 |
| WO1998000521A1 | 1998-01-08 |
JPN6012040507; Ｂｉｏｔｅｃｈｎｏｌ．Ｐｒｏｇ． Ｖｏｌ．１６, 2000, ｐ．６８８-６９２
Ishii Teiji
Satoshi Fujita
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