Title:
動物肝細胞の培養方法
Document Type and Number:
Japanese Patent JP5337169
Kind Code:
B2
Abstract:
Provided are a technique for easily forming a spheroid by three-dimensionally culturing hepatocytes, and a technique for forming a spheroid having a higher expression level of a transporter MRP2 playing a role of biliary excretion than that of a conventional method. In order to solve the above-described problems, the present inventors have found out a condition under which hepatocytes easily form the spheroid on a nanopillar sheet. More specifically, this is related to a concentration of Type I collagen coated onto the NP sheet. Also, they have found out a condition under which an expression level of a gene related to the excretion of the formed spheroid is improved. More specifically, after the spheroid is previously formed, a biological matrix is overlayered thereon.
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Inventors:
Ryosuke Takahashi
Akiko Hisada
Hiroshi Sonoda
Akiko Hisada
Hiroshi Sonoda
Application Number:
JP2010545661A
Publication Date:
November 06, 2013
Filing Date:
January 08, 2009
Export Citation:
Assignee:
株式会社日立製作所
International Classes:
C12N5/071
Domestic Patent References:
| JP2008199962A | 2008-09-04 | |||
| JP2004170935A | 2004-06-17 | |||
| JPH07274952A | 1995-10-24 |
Foreign References:
| WO2005047496A1 | 2005-05-26 |
Other References:
JPN6009012614; Ｊｏｕｒｎａｌ ｏｆ Ａｒｔｉｆｉｃｉａｌ Ｏｒｇａｎｓ Ｖｏｌ． ９, 2006, ｐ． ９０-９６
Attorney, Agent or Firm:
Yamato Tsutsui