Title:
アネキシンＶの製造プロセス
Document Type and Number:
Japanese Patent JP6910408
Kind Code:
B2
Abstract:
The present invention provides a process for the recovery and/or purification of a recombinantly expressed intracellular protein comprising the sequence of Annexin A5 (AnxA5) from an endotoxin-producing host cell with a cell wall, wherein the process comprises releasing the intracellular protein from the host cell, characterised in that the step of releasing the intracellular AnxA5 protein is conducted in the presence of a homogenisation buffer comprising non-ionic detergent, and preferably wherein the process does not include any centrifugation steps for the recovery and/or purification of the AnxA5 protein after its release from the host cell and/or in which the AnxA5 protein remains in solution throughout the process except when temporarily bound to any chromatographic resins.
Inventors:
Mox, thomas
Reich, Jan Christoph
Reich, Jan Christoph
Application Number:
JP2019192805A
Publication Date:
July 28, 2021
Filing Date:
October 23, 2019
Export Citation:
Assignee:
Annexin Pharmaceuticals Arb
International Classes:
C07K14/47; A61K38/16; A61L31/16; A61P7/02; A61P7/06; A61P9/00; A61P9/10; A61P9/12; A61P11/00; A61P19/02; A61P19/08; A61P21/04; A61P25/28; A61P29/00; A61P37/06; C07K1/18; C12P21/02
Domestic Patent References:
| JP2015519339A | ||||
| JP2012521977A | ||||
| JP2012505837A | ||||
| JP2008502592A |
Foreign References:
| EP0441274A1 |
Other References:
BMC Biotechnol.,2014年,14(33),1-9
Protein Expression and Purification,2006年,45,80-87
Protein Expression and Purification,2006年,45,80-87
Attorney, Agent or Firm:
Sonoda/Kobayashi Patent Business Corporation