Title:
抗CEA、抗CD3及び抗CD28の遺伝子組み換えによる糸状な単鎖の三重特異性抗体
Document Type and Number:
Japanese Patent JP4648382
Kind Code:
B2
Abstract:
This invention refers to a linear single chain recombinant trispecific antibody (scTsAb). This trispecific antibody is composed of an anti-carcinoembryonic antigen (CEA), an anti-CD3, and an anti-CD28 (CEA-scTsAb). More specifically, a Fc linking peptide fuses the anti-CEA single chain fragment (scFv) to the anti-CD3 scFv, and an HSA linking peptide fuses the anti CD3 scFv to the anti-CD28 heavy variable chain (V H ) in tandem. A c-myc-tag and/or (His) 6-tag is attached to the C terminal of the CEA-scTsAb either alternatively or simultaneously. This invention also refers DNA sequence coding for the said scTsAbs, plasmids containing the said sequences, and host cells containing the said plasmids. These invented scTsAbs are intended for use in the development of therapeutic drug candidate for treatment of human colon cancer.
Inventors:
Wang Sho
Yellow Flower Beam
Zhao Baofeng
Zhao Saki
Park Nishiki Hana
Hayashi Haru
Yellow Flower Beam
Zhao Baofeng
Zhao Saki
Park Nishiki Hana
Hayashi Haru
Application Number:
JP2007505359A
Publication Date:
March 09, 2011
Filing Date:
March 29, 2005
Export Citation:
Assignee:
Beijing Anba Special Factors Engineering Technology Co., Ltd.
Dong Gango Biological Process Techniques Kaikai Co., Ltd.
Dong Gango Biological Process Techniques Kaikai Co., Ltd.
International Classes:
C12N15/09; A61K39/395; A61P35/00; A61P43/00; C07K16/18; C07K16/28; C07K16/30; C07K16/46; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/12; C12N15/13; C12N15/63; C12N15/70
Foreign References:
| WO2002083738A1 |
Other References:
Cancer Res., vol. 59, pages 2909-2916 (1999)
Song, L.P., et al.,Acta Biochimica et Biophysica Sinica,2003年,Vol.35(6),p.503-10
Song, L.P., et al.,Acta Biochimica et Biophysica Sinica,2003年,Vol.35(6),p.503-10
Attorney, Agent or Firm:
Yasuo Itaya