PURPOSE: To obtain the title fibroin membrane useful for an immune sensor, capable of carrying out high-accuracy measurement free from variability by immobilizing an antibody or an antigen through covalent bond with glutaraldehyde, its polymer or cyanuric chloride, etc., as a bonding reagent.
CONSTITUTION: Raw silk is immersed in 1.0wt.% aqueous solution of Marseille soap, scoured at 80°C, washed with water, sericin, etc., are removed to give a fibroin raw material, which is dialyzed and desalted with flowing water, the aqueous solution of fibroin is mixed with 30wt.% based on fibroin of glycerol, the solution is cast into an acrylic plate having a divided square, dried at 20°C for 10 hours, made into a coating film and released. Then the film is immersed in 80% aqueous solution of methanol at room temperature for 3 minutes to give an insoluble silk fibroin membrane. An aqueous solution of an antibody or an antigen is brought into contact with the film by using glutaraldehyde, its polymer or cyanuric chloride, etc., as a bonding reagent and the antibody or the antigen is immobilized to the film through covalent bond to give a sink fibroin membrane.
NAGURA SATORU
NAKAYAMA HIROSHI
