Title:
ANTIGEN FOR IMMUNOASSAY OF ANTIHUMAN THYROID PEROXIDASE ANTIBODY AND RECOMBINANT HUMAN THYROID PEROXIDASE
Document Type and Number:
Japanese Patent JPH1194833
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide an antigen, for the immunoassay of an antihuman thyroid peroxidase(TPO) antibody, whose measuring sensitivity is higher than that in a case where a human TPO prepared by a genetic engineering technique by making use of a chinese hamster ovary(CHO) cell in conventional case as a host is used as an antigen when it is used as an antigen to measure the antihuman TPO. SOLUTION: An antigen is composed of a recombinat human TPO obtained in such a way that a nucleic acid in which a human TPO gene or one base or a plurality of bases of the gene are substituted or lost or in which one base or a plurality of bases are added to, or inserted into, the gene is expressed in an insect cell or of its derivative, and it performs an antigen-antibody reaction with an antihuman TPO antibody.
Inventors:
FUKUSHIMA MASAO
Application Number:
JP27374397A
Publication Date:
April 09, 1999
Filing Date:
September 19, 1997
Export Citation:
Assignee:
SRL KK
International Classes:
G01N33/53; C12N9/08; C12N15/09; G01N33/531; G01N33/564; C12R1/91; (IPC1-7): G01N33/53; C12N9/08; C12N15/09; G01N33/531; G01N33/564
Attorney, Agent or Firm:
Eijiro Tanigawa