Title:
ANTIMICROBIAL PEPTIDE AND ANTIMICROBIAL AGENT
Document Type and Number:
Japanese Patent JP3173858
Kind Code:
B2
Abstract:
PURPOSE: To obtain an antimicrobial peptide useful as a food additive, having no side effect, comprising a specific number of amino acid residues wherein amino acid residues of N-end and C-end are arginine and lysine and the rest consists of amino acid residues except cysteine.
CONSTITUTION: Bovine lactoferrin separated by a conventional procedure is hydrolyzed with an enzyme to produce an antimicrobial peptide. Otherwise, by a solid-phase peptide synthesis method using a peptide autosynthesizer device and an insoluble functional group-containing resin as a solid-phase carrier, an amino acid containing an amine functional group protected with 9- fluorenylmethoxycarbonyl (Fmoc) group is coupled to the resin, an amino- protecting group of C-end amino acid is removed, these operations are repeated to synthesize a peptide chain, the protecting group is removed and the peptide is released to give the objective antimicrobial peptide (derivative) containing an amino acid sequence comprising ≥6 amino acid residues wherein the amino acid residue of N-end or C-end is arginine or lysine residue and the other four residues are the amino acid residues excluding cysteine alone.
Inventors:
Mamoru Tomita
Kohzo Kawase
Mitsunori Takase
Wayne Bellamy
Tsuneharu Yamauchi
Hiroyuki Wakabayashi
Yukiko Tokita
Kohzo Kawase
Mitsunori Takase
Wayne Bellamy
Tsuneharu Yamauchi
Hiroyuki Wakabayashi
Yukiko Tokita
Application Number:
JP10493392A
Publication Date:
June 04, 2001
Filing Date:
April 23, 1992
Export Citation:
Assignee:
Morinaga Milk Industry Co., Ltd.
International Classes:
A01N37/44; A23L3/3526; A61K38/00; A61P31/04; C07K7/06; C11D3/48; C11D9/50; (IPC1-7): C07K7/06; A23L3/3526; A61K38/00
Domestic Patent References:
| JP6356273A |
Other References:
【文献】Nucleic Acids Res(1990),Vol.18,No.23,p.7167
【文献】Nucleic Acids Res(1990),Vol.18,No.13,p.4013
【文献】Nucleic Acids Res(1990),Vol.18,No.17,p.5288
【文献】Nucleic Acids Res(1990),Vol.18,No.13,p.4013
【文献】Nucleic Acids Res(1990),Vol.18,No.17,p.5288
Attorney, Agent or Firm:
Toshio Nishizawa
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