NEW MATERIAL:The antibiotic substance having the following properties: Solubility, soluble in water, insoluble in methanol, ethanol, acetone, ethyl acetate, and n-hexane; infrared absorption spectrum, FIG.1; ultraviolet absorption spectrum, FIG.2; specific rotation, [α]26D=-75.6° in 0.25% aqueous solution; melting point, decomposes at ≥240°C; elemental analysis, C 46.6%, H 6.45%, N 13.34%, S 0.20%, O 33.41%; molecular weight, a polymeric peptide having a molecular weight of about 22,000; color reactions, decolors potassium permanganate solution, positive to Folin-Lawry, xanthoprotein, biuret, and ninhydrin reactions, and negative to anthrone and Sakaguchi reactions.
USE: Antibiotic substance BBM-1644 active to inhibit the proliferation of gram- positive and acid-fast bacteria, and inhibit the progress of P388 leukemia of mouse.
PROCESS: A BMM-1644-producing strain of Actinomadura species [e.g. Actinomadura H-710-49 (ATCC-39144)] is cultured in an aqueous medium containing assimilable C and N sources in an aerated deep-tank fermentor.
SAKAI FUMIHIDE
MIYAGI TAKEO
KAWAGUCHI HIROSHI
