To provide an aqueous solution and a method for protein extraction, which are capable of extracting protein even from heated food without being denatured, and to provide an analyzing method for allergen.
An aqueous solution for extraction contains an alkyl sulfate, urea, and dithiothreitol or 2-mercaptoethanol in the molar ratios 3.1-52:2000 or more:1.1 or more (in the case of dithiothreitol) or 9-400 (in the case of 2- mercaptoethanol). The extraction is performed in an aqueous extraction system containing 0.09-1.5 wt. % of the alkyl sulfate, 2 M or more of urea, and 1.1 mM or more of dithiothreitol or 9-400 mM of 2-mercaptoethanol. Allergen analysis is carried out for immunologically measuring the allergenition of protein in the obtained extract.
IKEZAWA YOSHIRO
SHIMADA SADASUKE
MOGI KAZUYUKI
SUGIYAMA HIROSHI
