PURPOSE: To improve detection specificity by eliminating nonspecific reaction by mixing a chelating agent such as ethylenediamine 4 acetate to a specific concentration when measuring an antigen and antibody by tracing agglutination in an aqueous solvent.
CONSTITUTION: When a antigen or antibody based upon immune reaction and a sample are mixed in an aqueous solvent for coagulation test such as water and a solution of physiologic salt to take a measurement by tracing agglutination, a chelating agent such as ethylenediamine 4 acetate is added to the aqueous solvent to a concentration of 0.005W1M. Thus, nonspecific agglutination due to mixture of a pseudo reactant such as lipoprotein and difrinogen in the sample is eliminated to improve detection specificity and the need to remove the preudo reactant from the sample is eliminated.
FUKUYAMA KAZUMI
KAMIMURA YATSUHIRO
FUNAKOSHI SATORU
| JPS4971133A | 1974-07-10 |