PURPOSE: To obtain an ascorbic acid oxidase excellent in thermal stability.
CONSTITUTION: For example, 60ml culture medium containing 2% polypeptone, 2% malt extract and 0.2% yeast extract is transferred to a Sakaguchi flask of 500ml capacity and treated at 121°C for 15min in an autoclave. Volutella.sp. TE5082 (FERM P-13297) in an amount of one platinum loop as a spawn is inoculated and cultured at 25°C for 72hr to provide a seed culture solution. The resultant culture medium in a volume of 6L is subsequently transferred to a jar fermenter of 10L capacity, treated at 121°C for 15min in an autoclave and then allowed to cool. The obtained seed culture solution in a volume of 60ml is then transferred thereto and cultured at 25°C and 300r.p.m. for 48hr by aeration at 2L/min. The resultant culture solution is filtered by suction to remove microbial cells. Thereby, a culture supernatant is obtained. The production of the ascorbate oxidase is 1.9U per ml culture solution and 0.8U thereof is present in the culture supernatant.
SOGABE YUKIHIRO
AISUI SHIGENORI
