Title:
ASSAY REAGENT AND APPARATUS
Document Type and Number:
Japanese Patent JP3939403
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To enable a highly clear and accurate assay by a small amt. of a reagent by directly adding a particle label simultaneously sensitized to non- specific protein capable of being participated in the control reaction of a first specific binder and a separate specific binder.
SOLUTION: A particle label (e.g; latex particles having a proper size) is brought into contact with a mixture of two materials for sensitizing the particles in an aq. suspension to prepare a simultaneously sensitized particle label. For example, it is necessary to simultaneously sensitize a mixture of a mouse monoclonal antibody to the α-chain of hCG in a proper specific wt. ratio and a non-specific polyclonal antibody (e.g; rabbit IgG) under a buffering condition in the same way as the case of standard procedure. After a time interval sufficient to bond these materials on latex particles, the unbonded material is separated by a conventional method such as centrifugal separation and the simultaneously sensitized particles can be re-suspended in a form of a storage buffer soln. usuable in a test as it is.
Inventors:
Yvonne Elizabeth Penford
David Alan Percival
David Alan Percival
Application Number:
JP26152597A
Publication Date:
July 04, 2007
Filing Date:
September 26, 1997
Export Citation:
Assignee:
Inverness Medieval Sweets Tour Land Gezershyaft Mitz Besilenktel Haftung
International Classes:
G01N33/531; G01N33/558; G01N33/58; (IPC1-7): G01N33/531
Domestic Patent References:
| JP4064061A | ||||
| JP8094619A | ||||
| JP7191030A | ||||
| JP8505224A |
Attorney, Agent or Firm:
Yoshio Kawaguchi
Mitsuru Inoue
Akio Ichiiri
Katsuma Osaki
Takamasa Soma
Mitsuru Inoue
Akio Ichiiri
Katsuma Osaki
Takamasa Soma
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