PURPOSE: To perform assay with a high accuracy and in a short time by forming a polymer layer having a carboxyl group on the surface part of a filler.
CONSTITUTION: The mixed liquid of a hydrophobic monomer is added into a water phase wherein suspension stabilizer is dispersed. After the substitution with nitrogen, the liquid is agitated and heated, and suspension polymerization is performed. When diluent is added and the polymerization is performed, organic solvent is removed after the end of the polymerization, and porous spherical particles are obtained. Then, the hydrophobic cross link polymer is impregnated with a polymerization initiator. The particles are dispersed in a dispersion medium wherein a monomer having a hydrolytic group is dissolved. After the substitution with hydrogen, the material is agitated and heated, and polymerization is performed. Thus, the polymer particles having a double-layered structure are obtained. Then, the polymer particles are washed and undergo hydrolysis with acid catalyst or alkali catalyst. The functional group on the coating layer on the surface of the particle is made to be a carboxyl group. The particles are filtered, washed with water and dried. Thus a filler is obtained. A column is filled with the filler. A sample blood is hemolyzed and applied on the column. Thus, saccharified hemoglobin can be assayed by liquid chromatography.
TAKECHI MASAHIRO
YAMAZAKI KAZUTOSHI
| JPS58760A | 1983-01-05 | |||
| JPS60213863A | 1985-10-26 | |||
| JPS62277149A | 1987-12-02 |