Title:
リアルタイム核酸増幅の自動分析
Document Type and Number:
Japanese Patent JP4689004
Kind Code:
B2
Abstract:
A method is described for analyzing a sample for the presence of a nucleic acid wherein the sample is amplified, preferably using PCR, in the presence of a fluorescent probe capable of detecting the presence of the nucleic acid sample. A baseline region is determined by comparing the fluorescence at various amplification cycles, and the fluorescence at each of various amplification cycles is compared to the baseline region to determine whether the fluorescence measurements fall outside of that baseline region.
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Inventors:
Karl Tee. Witter
Application Number:
JP2000128560A
Publication Date:
May 25, 2011
Filing Date:
April 27, 2000
Export Citation:
Assignee:
UNIVERSITY OF UTHA RESEARCHFOUNDATION
International Classes:
C12Q1/68; C12N15/09; G01N21/64; G01N33/50; G01N33/58; G01N35/00
Foreign References:
| WO1997046714A1 | ||||
| WO1997046708A1 |
Other References:
GERARD, C.J. et al.,"Improved quantitation of minimal residual disease in multiple myeloma using real-time polymerase chain reaction and plasmid-DNA complementarity determining region III standards.",CANCER RES.,1998年 9月 1日,Vol.58, No.17,P.3957-3964
LOVATT, A. et al.,"High throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods.",J. VIROL. METHODS,1999年10月,Vol.82, No.2,P.185-200
LOVATT, A. et al.,"High throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods.",J. VIROL. METHODS,1999年10月,Vol.82, No.2,P.185-200
Attorney, Agent or Firm:
Kensuke Isshiki
Noritaka Harashima
Satoshi Suzuki
Noritaka Harashima
Satoshi Suzuki