Title:
抗酸菌の溶菌方法およびそれを用いた遺伝子増幅若しくは検出方法
Document Type and Number:
Japanese Patent JP3910198
Kind Code:
B2
Abstract:
A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96 DEG C for 10 min. As the nonionic surfactant use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.
Inventors:
Tatsuo Kamada
Yuji Izumisawa
Yuji Izumisawa
Application Number:
JP2004506475A
Publication Date:
April 25, 2007
Filing Date:
May 21, 2003
Export Citation:
Assignee:
Arkray Co., Ltd.
International Classes:
C12N1/06; C12N15/10; C12Q1/68
Domestic Patent References:
| JP10304869A | ||||
| JP6319527A | ||||
| JP9505479A |
Attorney, Agent or Firm:
Ikeuchi, Sato & Partners