BACTERIUM-DETECTING OLIGONUCLEOTIDE PROBE BELONGING TO GENUS LEGIONELLA AND DETECTION OF THE SAME BACTERIUM

Japanese Patent JP3814909

B2

PROBLEM TO BE SOLVED: To provide an oligonucleotide probe for detecting a genus Legionella bacterium, capable of specifically detecting various species of genus Legionella bacteria with high accuracy by one kind of probe in a short time and capable of easily preparing, and to specifically detect various species of genus Legionella bacteria with high accuracy in a short time by using the probe.
SOLUTION: A bacterium belonging to genus Legionella is detected by performing hybridization by bringing a label oligonucleotide probe obtained by labeling an oligonucleotide probe having a base sequence of 5'AGC AAA CGC GAT AAG TTG AC3' with a radioactive element, an enzyme, a fluorescent substance or a chemical substance into contact with a bacterium belonging to genus Legionella or its DNA, and using the label as an indicator.

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Wakako Sato
Takayuki Esaki
Hiroyuki Yamamoto

JP505097A

August 30, 2006

January 14, 1997

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Kurita Water Industries Ltd.

C12N15/09; C12Q1/04; C12Q1/68; C12R1/01; (IPC1-7): C12N15/09; C12Q1/04; C12Q1/68; //(C12Q1/68; C12R1:01)

Manz W. et al.,In situ identification of legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy.,Microbiology,1995年,Vol. 141,p. 29-39

Shigeru Yanagihara

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