BETA-CHAIN CONSTANT-REGION POLYPEPTIDE OF MAMMALIAN T-CELL RECEPTOR

Title:

BETA-CHAIN CONSTANT-REGION POLYPEPTIDE OF MAMMALIAN T-CELL RECEPTOR

Document Type and Number:

Japanese Patent JPS63276496

Kind Code:

A

Abstract:

PURPOSE: To supply a mammalian T-cell receptor in a large amount, by cultivating a microorganism containing a recombinant DNA, having a DNA capable of coding a β-chain constant-region polypeptide of a specific mammalian T-cell receptor and integrated thereinto.

CONSTITUTION: A microorganism containing a recombinant DNA having a DNA capable of coding a β-chain constant region polypeptide of a mammalian T-cell receptor expressed by formula I is cultivated. A polypeptide is accumulated in the resultant culture by this cultivation and then the aimed β-chain constant-region polypeptide of the mammalian T-cell receptor is collected. In this case, a plasmid having a tryptophan promoter is used and the DNA is preferably integrated into the downstream side of the tryptophan promoter. Furthermore, a microorganism belonging to Escherichia coli is preferred as the microorganism.

Inventors:

KUWANA YOSHIHISA
KUROSAWA YOSHIKAZU
ITOU SEIGA

Application Number:

JP11113187A

Publication Date:

November 14, 1988

Filing Date:

May 07, 1987

Export Citation:

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Assignee:

KYOWA HAKKO KOGYO KK
FUJITA GAKUEN

International Classes:

C12N15/09; A61K39/395; A61P35/00; C07K14/00; C07K14/705; C07K14/725; C07K16/00; C12N1/20; C12N15/02; C12P21/02; C12P21/08; C12R1/19; (IPC1-7): A61K39/395; C07K13/00; C12N1/20; C12N15/00; C12P21/00; C12P21/02; C12R1/19

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