Title:
バイオニック氷制御材料及びこのバイオニック氷制御材料を含有する凍結保存液
Document Type and Number:
Japanese Patent JP7389138
Kind Code:
B2
Abstract:
The present invention relates to a biomimetic ice growth inhibition material, which is prepared by constructing a library for structures of compound molecules, with the compound molecules comprising a hydrophilic group and an ice-philic group, by evaluating the spreading performance of each compound molecule at an ice-water interface by adopting molecular dynamics simulation (MD simulation), and by screening the compound molecules with the desired affinities for ice and water. The present invention firstly provides the mechanism of the affinities of the ice growth inhibition material for ice and water, introduces MD simulation into the molecular structure design of the ice growth inhibition material, evaluates the affinities of the designed ice growth inhibition material for ice and water through MD simulation, predicts the ice growth inhibition performance of the ice growth inhibition material, and can realize the optimization of the structure. The biomimetic ice growth inhibition material designed and screened by the method disclosed herein has good capability of inhibiting the growth of ice crystals, and can achieve a good cryopreservation effect on reagents even without adding DMSO.
Inventors:
Wang Ken
Kim Jin Lin
Lu Kenyu
Strict
Takashi
Enrei
Kim Jin Lin
Lu Kenyu
Strict
Takashi
Enrei
Application Number:
JP2021560635A
Publication Date:
November 29, 2023
Filing Date:
March 02, 2020
Export Citation:
Assignee:
INSTITUTE OF CHEMISTRY, CHINESE ACADEMY OF SCIENCES
Beijing University Third Clinic (Beijing University Third Clinical Clinic)
Beijing University Third Clinic (Beijing University Third Clinical Clinic)
International Classes:
G01N33/00
Domestic Patent References:
| JP11509832A |
Foreign References:
| US20150157010 | ||||
| US20100068692 |
Other References:
WENG Lindong et al.,Molecular Dynamics at the Interface between Ice and Poly(vinyl alcohol) and Ice Recrystallization Inhibition,Langmuir,2018年,Vol.34,pp.5116-5123
MOCHIZUKI Kenji and MOLINERO Valeria,Antifreeze Glycoproteins Bind Reversibly to Ice via Hydrophobic Groups,Journal of American Chemical Society,2018年,Vol.140,pp.4803-4811
NAULLAGE Pavithra M. et al.,Molecular Recognition of Ice by Fully Flexible Molecules,The Journal of Physical Chemistry C,2017年,Vol.121,pp.26949-26957
MOCHIZUKI Kenji and MOLINERO Valeria,Antifreeze Glycoproteins Bind Reversibly to Ice via Hydrophobic Groups,Journal of American Chemical Society,2018年,Vol.140,pp.4803-4811
NAULLAGE Pavithra M. et al.,Molecular Recognition of Ice by Fully Flexible Molecules,The Journal of Physical Chemistry C,2017年,Vol.121,pp.26949-26957
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK