Title:
生物学的分子を検出および定量するためのバイオセンシングプラットホーム
Document Type and Number:
Japanese Patent JP2005501248
Kind Code:
A
Abstract:
A bioconjugate comprising a fluorescer (P) linked to a quencher (Q) by a tether (T) is provided. The tether (T) includes a segment that can recognize and interact with a target biomolecule. In the absence of a specific interaction of the bioconjugate with an enzyme or other target biomolecule recognizing the bioconjugate, the fluorescence of the polymer (P) is attenuated or modified by the relatively close proximity thereto of the quencher (Q). As a consequence of the association of the bioconjugate with the target biomolecule, a reaction can occur which results in a cleavage of the bioconjugate tether and a release of the fluorescent polymer and/or quencher. This sequence of events can be followed by an enhancement or amplification of the polymer fluorescence.
Inventors:
Coomaras Whame, Sri Ram
Whitten, david
McBranch, Duncan
Linins Land, Flok
Birdic, Brent Arthur
Whitten, david
McBranch, Duncan
Linins Land, Flok
Birdic, Brent Arthur
Application Number:
JP2003523958A
Publication Date:
January 13, 2005
Filing Date:
August 23, 2002
Export Citation:
Assignee:
Cut L Biosystems Limited Liability Company
International Classes:
C12Q1/34; C12Q1/42; C12Q1/48; C12Q1/68; C12Q1/6818; C12Q1/6823; G01N21/78; G01N33/53; G01N33/533; G01N33/542; G01N33/566; G01N33/573; G01N33/58; (IPC1-7): G01N33/566; C12Q1/34; C12Q1/42; C12Q1/48; C12Q1/68; G01N21/78; G01N33/53; G01N33/533; G01N33/573; G01N33/58
Attorney, Agent or Firm:
Kuro Fukami
Toshio Morita
Yoshihei Nakamura
Yutaka Horii
Hisato Noda
Masayuki Sakai
Toshio Morita
Yoshihei Nakamura
Yutaka Horii
Hisato Noda
Masayuki Sakai