Title:
ハイブリダイゼーション用バッファー組成物及びハイブリダイゼーション方法
Document Type and Number:
Japanese Patent JP6757942
Kind Code:
B2
Abstract:
In the case of using a blocking nucleic acid to prevent non-specific hybridization of a target nucleic acid with a nucleic acid probe, further excellent efficiency of detecting the target nucleic acid is achieved. A buffer composition used in hybridization of a target nucleic acid with a nucleic acid probe, wherein the buffer composition for hybridization contains a blocking nucleic acid comprising a nucleotide sequence complementary to a region comprising at least a non-detection target nucleotide in a non-target nucleic acid, in a concentration of one or more times higher than the concentration of a nucleic acid in a nucleic acid mixture consisting of the target nucleic acid and the non-target nucleic acid.
Inventors:
Toshiya Tsuda
Shuichi Kamei
Mitsuyoshi Oba
Hirofumi Yamano
Ryoichi Tsunetomi
硲 彰一
Hiroaki Nagano
Shuichi Kamei
Mitsuyoshi Oba
Hirofumi Yamano
Ryoichi Tsunetomi
硲 彰一
Hiroaki Nagano
Application Number:
JP2016153058A
Publication Date:
September 23, 2020
Filing Date:
August 03, 2016
Export Citation:
Assignee:
TOYO KOHAN CO.,LTD.
Yamaguchi University
Yamaguchi University
International Classes:
C12Q1/6813; C12N15/00
Domestic Patent References:
| JP2010200701A |
Foreign References:
| WO2015045741A1 |
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Satoshi Fujita