Title:
Butyric acid-producing bacteria and their use
Document Type and Number:
Japanese Patent JP6357222
Kind Code:
B2
Abstract:
Provided is a novel butyrate-producing bacterium. A butyrate-producing bacterium belonging to Anaerostipes hadrus , wherein the amount of butyrate produced which is measured by thawing a frozen stock solution of the bacterial strain (a 10% (w/v) skim milk-2% sodium glutamate solution in which bacterial cells are suspended) (cell count: 2.0 to 5.5 × 10 10 cells/mL), inoculating 1the solution at 1% to 4 mL of a PY liquid medium supplemented with 33 mM sodium acetate and 0.5 (w/v)% glucose (PYGA medium), followed by anaerobic culture at 37°C for 24 hours, then inoculating the culture solution at 1% to a PYGA medium, followed by anaerobic culture at 37°C for 24 hours, then inoculating the culture solution at 1% to a PY medium containing 33 mM sodium acetate and 0.5 (w/v)% L-sorbose, followed by anaerobic culture at 37°C for 24 hours, and then measuring the butyrate concentration in the culture solution is at least 1.5 times that of Anaerostipes hadrus ( Eubacterium hadrum ) YIT 10092 T (DSM 3119 T ), which is a type strain of Anaerostipes hadrus ( Eubacterium hadrum ) .
Inventors:
Nao Sato
Shiro Kusuhara
Shiie Yokoi
Masahiko Ito
Koji Miyazaki
Akira Kushiro
Shiro Kusuhara
Shiie Yokoi
Masahiko Ito
Koji Miyazaki
Akira Kushiro
Application Number:
JP2016510558A
Publication Date:
July 11, 2018
Filing Date:
March 27, 2015
Export Citation:
Assignee:
Yakult Honsha Co., Ltd.
International Classes:
A23K10/18; C12N1/20; A23K20/163; A23L33/135; A61K31/047; A61K31/70; A61K31/7004; A61K31/7016; A61K31/702; A61K35/741; A61P43/00
Domestic Patent References:
| JP2009084215A |
Foreign References:
| WO2007114378A1 |
Other References:
ALLEN-VERCOE E et al., Anaerostipes hadrus comb. nov., a dominant species within the human colonic microbiota; reclassification of Eubacterium hadrum Moore et al. 1976, Anaerobe, 2012, Vol.18, p.523-529
BUI TPN et al., Anaerostipes rhamnosivorans sp. nov., a human intestinal, butyrate-forming bacterium, Int. J. Syst. Evol. Microbiol. [online], 2014.03.01, [retrieved on 2015.06.15], Vol.64, p.787-793,
SCHWIERTZ A et al., Anaerostipes caccae gen. nov., sp. nov., a new saccharolytic, acetate-utilising, butyrate-producing bacterium from human faeces, Syst. Appl. Microbiol., 2002, Vol.25, p.46-51
佐藤直他, 乳酸を酪酸に変換するヒト腸内細菌のin vivoにおける酪酸産生能力の検証, 日本細菌学雑誌, 2006, Vol.61, p.138, III-E-13
BUI TPN et al., Anaerostipes rhamnosivorans sp. nov., a human intestinal, butyrate-forming bacterium, Int. J. Syst. Evol. Microbiol. [online], 2014.03.01, [retrieved on 2015.06.15], Vol.64, p.787-793,
SCHWIERTZ A et al., Anaerostipes caccae gen. nov., sp. nov., a new saccharolytic, acetate-utilising, butyrate-producing bacterium from human faeces, Syst. Appl. Microbiol., 2002, Vol.25, p.46-51
佐藤直他, 乳酸を酪酸に変換するヒト腸内細菌のin vivoにおける酪酸産生能力の検証, 日本細菌学雑誌, 2006, Vol.61, p.138, III-E-13
Attorney, Agent or Firm:
Alga Patent Office, a patent business corporation
Toshio Takano
Toshio Nakajima
Masaki Murata
Hiroto Yamamoto
Toshio Takano
Toshio Nakajima
Masaki Murata
Hiroto Yamamoto