PURPOSE: To obtain a cardiotonic agent, available from a culture prepared culturing a sporogenous imperfect fungus which belongs to the genus Trichothecium and capable of producing a cyclic depsipeptide, having strong cardiotonic actions without causing side effects such as tachycardia or arrhythmia and useful for treating, etc., congestive heart failure.
CONSTITUTION: This cardiotonic agent excellent in cardiotonic effects is obtained by inoculating a sporogenous imperfect fungus, belonging to the genus Trichothecium and capable of producing a cyclic depsipeptide expressed by the formula (R is H or methyl) [e.g. Trichothecium TT103 strain (FERM P-13868)] into a culture medium, culturing the imperfect fungus at 25°C for 7 days, inoculating a spore formed on a hypha into a culture medium, culturing the inoculated spore at 25°C for 4 days by turning, subsequently filtering the resultant culture by suction, collecting a filtrate, passing the collected filtrate through an adsorbent resin column, then eluting the adsorbed substance with methanol, concentrating the obtained eluate, purifying the concentrate by silica get chromatography and high-performance liquid chromatography and providing a pharmaceutical preparation from the obtained compound as an active ingredient together with an excipient, a binder, a dispersing agent, etc.
TAKETOMO TADAO
YOSHIYAMA YOSHIHIRO
SATO YOSHIRO
AJISAKA KATSUMI
FUNAHASHI NORIO
KAMIJO MASAYUKI
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