CARRIER SUPPORTING MOLECULE, MANUFACTURE AND USE

Title:

CARRIER SUPPORTING MOLECULE, MANUFACTURE AND USE

Document Type and Number:

Japanese Patent JPS61130305

Kind Code:

A

Abstract:

1. Oligonucleotides or polynucleotides fixed on a solid support, characterised in that they comprise at least a structure of formula : see diagramm : EP0174879,P18,F1 in which : N represents a base such as uracil, methyl-5-uracil, guanine, xanthine, methyl-5-cytosine, cytosine, amino-2-adenine, hypoxanthine, S represents a ribose or a deoxy-2'-ribose moiety with R1 representing an OH group, a phosphate or even an oligo- or polynucleotide, R2 a hydrogen atom or an OH group and R3 an OH group, a phosphate or even an oligo- or polynucleotide, the numbers (2'), (3') and (5') corresponding to the positions of the sugar residues in question, numbered in the accepted manner, the oligo- or polynucleotide sequences being or not complementary and forming a double strand or the oligonucleotide fixed on the primer may be bent into a loop by forming a double strand and being fixed on the primer by one of the nucleotides of the loop, X represents a NH or O divalent group fixed on the base N at the level of the positions 4 or 6 respectively for a pyrimidine base and for a purine base, B represents a carbon chain of 4 to 20 carbon atoms, and A represents a solid support, n represents the number of chains fixed on a same support A, said chains may be different from one another and fixed by identical or different arms on the support, with the proviso that when N represents an adenin and one of the group R1 and R3 represent an -OH group and X represents NH, B be different from a dodecylamine radical.

Inventors:

TAMU YUANNDAN
SHIRUBUI POSHIE
JIYAN IGORAN

Application Number:

JP15394185A

Publication Date:

June 18, 1986

Filing Date:

July 12, 1985

Export Citation:

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Assignee:

PASTEUR INSTITUT
CENTRE NAT RECH SCIENT

International Classes:

C07H19/06; C07H19/16; C07H21/00; C07H21/04; C08B15/06; C08B37/00; C08F8/30; C08F8/40; C08F8/00; C12Q1/68; C12Q1/6834; G01N33/52; (IPC1-7): C07H19/06; C07H19/16; C07H21/00; C08B15/06; C08B37/00; C08F8/30; C08F8/40; G01N33/52

Attorney, Agent or Firm:

Yoshio Kawaguchi

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